LOCUS       sequence-79767-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-tagged mouse Arp11 under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Eckley DM, Schroer TA
  TITLE     Interactions between the evolutionarily conserved, actin-related 
            protein, Arp11, actin, and Arp1.
  JOURNAL   Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20.
  PUBMED    12857853
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2003-07"; volume: "14(7)"; pages: "2645-54. Epub 2003
            Mar 20"
COMMENT     Sequence Label: T7-His-GFP-Arp11
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..101
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(105..113,114..128)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(159..161,162..164,165..875)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(159..161,162..164,165..875)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(204..225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(465..484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     812..833
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc
       61 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg
      121 acgataagga tccgagctcg agatctgcag ctggtaccat ggtgagcaag ggcgaggagc
      181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      361 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      601 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      661 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      721 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      781 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      841 ccgggatcac tctcggcatg gacgagctgt acaagtccgg ccggactcag atctcgaggc
      901 caagaattcg gcacgaggca aagatgccgc tctacgaggg ccttgggagc ggcggcgaga
      961 agacagcagt cgtgatcgac ctag
//
