LOCUS       sequence-7977-ex        9321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GPD-EGFP-ccdB
FEATURES             Location/Qualifiers
     source          1..9321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2804..3448
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3342..3365
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     3454..3470
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3454..3470
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(3483..3485,3486..3488,3489..4199)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3483..3485,3486..3488,3489..4199)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3528..3549)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3789..3808)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4136..4157
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4224..4348
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4373..4378,4379..4396,4397..4403)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4457..5116
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4523..4542)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5461..5766
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5645..5664
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5807..5931)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5938..6185
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5954..5972)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6203..6222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6204..6222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6232..6249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6232..6248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6241..6263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6393..6848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6480..6499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6690..6711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7148..7552
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7565..8659
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9152..9171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9271..9293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctcttcgagt ttatcattat caatactcgc
     2821 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa
     2881 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac
     2941 agaatatata acaccgaagg tgcctgggtg aacaggttta ttcctggcat ccactaaata
     3001 taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata
     3061 ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac
     3121 aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga
     3181 gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt
     3241 ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc
     3301 cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa
     3361 ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta
     3421 gttttaaaac accagaactt agtttcgacg gattctagaa ctagtggatc ccccgggaca
     3481 aaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3541 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3601 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3661 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3721 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3781 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3841 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3901 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3961 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4021 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4081 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4141 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     4201 gtggaccagg tggtggacat caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     4261 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     4321 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     4381 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     4441 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     4501 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     4561 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     4621 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     4681 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4741 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     4801 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     4861 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     4921 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     4981 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     5041 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     5101 gcagggcggg gcgtaaacgc cgcgtggatc cggcttacta aaagccagat aacagtatgc
     5161 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     5221 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     5281 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     5341 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     5401 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
     5461 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     5521 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     5581 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     5641 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     5701 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     5761 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     5821 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     5881 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttcgatgtca
     5941 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
     6001 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     6061 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     6121 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     6181 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6361 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
     6421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     6841 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6901 tcgacggtcg aggagaactt ctagtatatc cacataccta atattattgc cttattaaaa
     6961 atggaatccc aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact
     7021 tccttgttca tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca
     7081 acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac
     7141 cgcagttaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca
     7201 accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa
     7261 attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc
     7321 aactgaaaaa ttgggagaaa aaggaaaggt gagaggccgg aaccggcttt tcatatagaa
     7381 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt
     7441 aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt
     7501 accttttaca tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc
     7561 ccctatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat
     7621 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt
     7681 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg tcccacttcc
     7741 agatgaggcg ctggaagcct ccaagaaggt tgatgccgtt ttgttaggtg ctgtgggtgg
     7801 tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga
     7861 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt
     7921 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt
     7981 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag
     8041 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct
     8101 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc
     8161 aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt
     8221 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa
     8281 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat
     8341 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac
     8401 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt
     8461 tgaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc
     8521 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac
     8581 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt
     8641 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
     8701 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
     8761 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatagtaa
     8821 aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca
     8881 ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa
     8941 atcatgaaac tacgattcct aatttgatat tggaggattt tctctaaaaa aaaaaaaata
     9001 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct
     9061 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     9121 ttacgacgta gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9181 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9241 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9301 cgtcatcacc gaaacgcgcg a
//
