LOCUS       sequence-79787-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-Dendra2 from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-Dendra2.W
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             148..525
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
       61 atcattttgg caaagaattg atttgatacc gcgggcccgg gatctcgagc tcaagcttcg
      121 aattctgcag tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg
      181 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
      241 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      301 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      361 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      421 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      481 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcg atccatgaac
      541 accccgggaa ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac
      601 gtgaacggcc acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc
      661 cagaccgcca acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg
      721 accaccgccg tgcactacgg caaccggg
//
