LOCUS       sequence-79893-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 V729C mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  TITLE     Forbush Lab human NKCC1 NT clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 V729C (NT503)
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgtccacag atcatcaaac caacattttt tgtgaaatcg tgaacaagat gaagtaaggc
       61 cggccgtgag tttggcgctc ctgtcataac aagacactgt ggcctaaagt ttttcacatg
      121 gtcctccacg ccagaaagcc taatactatg ttgcagggca ttgaggtaag tcagggcttg
      181 tgtagaggat ccccaattca catcgggttt tttgtaggta acataaatat agagaccaag
      241 gactatcacg taggttagca aagcagccca ccagttaata cagaacatta ctatgcaaca
      301 aagaattgct ccaagaagtg agatccacat attgtagtat ttaaatgccg gtcgccatcc
      361 tgggcttttt gccagtgaag catggaatac tgaaaaattg atgagggcat agctggcaag
      421 gaagaagttt gagataattg gtgcaataac attcagttcg gcaattaaga tgaatccaag
      481 tgcaattaag aatgttaaga tgtagccacg taatggctca ttatttttcc cataaccttt
      541 agcaaacatc tggaaagcgg ggtagatgtt gtctttacat agggcctgaa atatcttggg
      601 agcactcact agggatgcta atgcgctaga aagagtggca gagaatatgc ccgcggaaat
      661 tagtggtgta aatcctgaca ccatactcat tacctgaaag ttgttcatta ggccgtagct
      721 acaaggactg gattcacaag atgaaaaatc aaagtttaat ttgcaggctg cgctagtaca
      781 gtttgttagc tctgttacga tagtgtcatt aacattacca gtggcatcgc gtacgacaca
      841 agaacctaca gacacggcaa ttcctacata aaccaatgta gtaattaaaa tggctaggag
      901 tgttcctttg ggtatggctg actgaggatc tgcaagatca cctgagatat ttgcgccagc
      961 cagaattcca gttgcagcag gaaaaa
//
