LOCUS       sequence-79916-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of the site-specific recombinase Dre and BFP under the
            control of the Thy1.2 expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: Thy1.2-NLS_HA_Dre-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..770)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(771..827)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aggacttggg gagggagtca gctgaccggg agaagcaaga cctctgcaga ggaaggacct
       61 cgagctctga cctaattcag cttgtgcccc agtttgctag ggaggtcgca gtatctggcc
      121 actgccacct cgtgctgctc gacgtaggtc tcgttgttgg cctccttgat tctttccagt
      181 ctgtagtcca catagtagac gccaggcatc ttgaggttct tagcgggttt cttggatcta
      241 tatgtggtct tgatgtttgc gatcagatgg ctcccgccca cgagcttcag ggccatgtcg
      301 tttctgcctt ccaggccgcc gtcagcgggg tacagggtct cggtgaaggc ctcccagccg
      361 agtgttttct tctgcatcac agggccgttg gatgtgaagt tcacccctct gatcttgacg
      421 ttgtagatga ggcagccgtc ctggaggctg gtgtcctggg tagcggtcag cacgcccccg
      481 tcttcgtatg tggtgactct ctcccatgtg aagccctcag ggaaggactg cttgaagaag
      541 tcggggatgc cctgggtgtg gttgatgaag gtcttgctgc cgtagaggaa gctagtagcc
      601 aggatgtcga aggcgaaggg gagagggccg ccctcgacca ccttgattct catggtctgg
      661 gtgccctcgt agggcttgcc ttcgccctcg gatgtgcact tgaagtgatg gttgtccacg
      721 gtgccctcca tgtacagctt catgtgcatg ttctccttaa tcagctcgct gggaccgggg
      781 ttttcttcca cgtcgcctgc ttgctttaac agagagaagt tcgtggcgcc gctgccgcta
      841 tccatcagtc gagaattggc tcccacggac actccgcccc ttctgagata ccgcatcagt
      901 gtttc
//
