LOCUS       sequence-79936-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     Swiatecka-Urban Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cin85-3SH3-pCMV6-AN-GFP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..786
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 agctcgttta gtgaccgtca gaattttgta atacgactca ctatagggcg gccgggaatt
       61 cgtcgactgg atccgaggag atctgccacc atggagagcg acgagagcgg cctgcccgcc
      121 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
      181 ggcggagagg gcacccccga gcagggccgc atgaccaaca agatgaagag caccaaaggc
      241 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
      301 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
      361 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
      421 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtga tgggcaccgg cttccccgag
      481 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
      541 cccatgggcg ataacgatct ggatggcagc ttcacccgca ccttcagcct gcgcgacggc
      601 ggctactaca gctccgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
      661 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagga tcacagcaac
      721 accgagctgg gcatcgtgga gtaccagcac gccttcaaga ccccggatgc agatgccggt
      781 gaagaaagag cgatcgccat ggtggaggnc atagtggagt ttgactacca ngcccagcac
      841 gatgatgagc tgacgatcag c
//
