LOCUS       sequence-79939-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, 
            Tabar V, Mo Q, Studer L, Sadelain M
  TITLE     Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and 
            c-Myc expression for efficient human iPSC induction and 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
  PUBMED    19549847
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Aug 4. 106(31):12759-64."
COMMENT     Sequence Label: pLM-mCherry-Klf4
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     179..201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     193..210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     194..210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        220..238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      259..394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    434..555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(445..464)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     499..518
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
       61 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      121 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      181 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
      241 gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
      301 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
      361 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggatagcta
      421 gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      481 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      541 tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt ataaatactg
      601 aataataaga tgacatgaac tactactgct agagattttc cacactgact aaaagggtct
      661 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
      721 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gct
//
