LOCUS       sequence-79979-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hCas9 under the CAG promoter for CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Horii T, Tamura D, Morita S, Kimura M, Hatada I
  TITLE     Generation of an ICF syndrome model by efficient genome editing of 
            human induced pluripotent stem cells using the CRISPR system.
  JOURNAL   Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 
            10.3390/ijms141019774.
  PUBMED    24084724
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Int J Mol Sci"; date: "2013-09-30- 30"; volume: "14"; issue: "10"; 
            pages: "19774-81"
COMMENT     Sequence Label: pCAG-hCas9
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(141..161)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctgcataa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tctagagtcg cggccgctca caccttcctc ttcttcttgg ggtcagccct gctgtctcca
      181 ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg atgaatcagt
      241 gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag
      301 tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat aatgttttct
      361 gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac cttatcgagg
      421 ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat gatctcatca
      481 aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc gggagaccct
      541 ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga gggcagtgcc
      601 agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg gccgttttca
      661 agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac ctctttatat
      721 cctttcgcct cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc catgattgtg
      781 atgcccagca gttccttgac gcttttgagt tttttagact tccctttctc cactttggcc
      841 acaaccagta cactgtaagc gactgtagga gaatcgaatc cgccgtattt cttggggtcc
      901 caatcttttt tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat actttccttg
      961 g
//
