LOCUS       sequence-79980-e         470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hCas9 under the CAG promoter for CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Horii T, Tamura D, Morita S, Kimura M, Hatada I
  TITLE     Generation of an ICF syndrome model by efficient genome editing of 
            human induced pluripotent stem cells using the CRISPR system.
  JOURNAL   Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 
            10.3390/ijms141019774.
  PUBMED    24084724
REFERENCE   2  (bases 1 to 470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Int J Mol Sci"; date: "2013-09-30- 30"; volume: "14"; issue: "10"; 
            pages: "19774-81"
COMMENT     Sequence Label: pCAG-hCas9
FEATURES             Location/Qualifiers
     source          1..470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(351..370)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(432..454)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcctatggaa gaaagagtca tccaccttag ccatctcatt actaaagatc tcctgcaggt
       61 agcagatccg attctttctg cgggtatatc tgcgccgtgc tgttcttttg agccgcgtgg
      121 cttcggccgt ctccccggag tcgaacagga gggcgccaat gaggttcttc tttatgctgt
      181 ggcgatcggt attgcccaga actttgaatt ttttgctcgg caccttgtac tcgtccgtaa
      241 tgacggccca gccgacgctg tttgtgccga tatcgagccc aatggagtac ttcttgtcca
      301 tggtggcggt accgtcgact gcagaattct ttgccaaaat gatgagacag cacaataacc
      361 agcacgttgc ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct
      421 ctagagccgc cggtcacacg ccagaagccg aaccccgccc tgccccgtcc
//
