LOCUS       sequence-80001-e        1968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive 
            Ca2+ indicator with Kd = 30 nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura
            T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
  TITLE     Spontaneous network activity visualized by ultrasensitive Ca(2+) 
            indicators, yellow Cameleon-Nano.
  JOURNAL   Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 
            2010 Aug 8.
  PUBMED    20693999
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1488"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "729-32"
COMMENT     Sequence Label: Yellow Cameleon-Nano30/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1147..1224
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             1231..1965
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgcccgcatg catgaccaac tgacagaaga gcagattgca
      721 gagttcaaag aagccttctc attattcgac aaggatgggg acggcaccat caccacaaag
      781 gaacttggca ccgttatgag gtcgcttgga caaaacccaa cggaagcaga attgcaggat
      841 atgatcaatg aagtcgatgc tgatggcaat ggaacgattt actttcctga atttcttact
      901 atgatggcta gaaaaatgaa ggacacagac agcgaagagg aaatccgaga agcattccgt
      961 gtttttgaca aggatgggaa cggctacatc agcgctgctg aattacgtca cgtcatgaca
     1021 aacctcgggg agaagttaac agatgaagaa gttgatgaaa tgataaggga agcagatatc
     1081 gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc aaaggggggc
     1141 gggtccaaga ggcgctggaa gaaaaacttc attgccgtca gcgctgccaa ccggttcaag
     1201 aagatctcca gctccggggc actggagctc atggacggcg gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggtggcagc
     1441 ggtggcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1561 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     1621 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     1681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1861 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     1921 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgagtaa
//
