LOCUS       sequence-80028-e        2266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent ATP 
            indicator, targeted to chloroplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: CT-Nano-lantern(ATP1)/pRSETB
FEATURES             Location/Qualifiers
     source          1..2266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(218..220,221..223,224..736)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(263..284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(524..543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     871..892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagaatg gattcacagc tagtcttgtc tctgaagctg aatccaagct tcactcctct
       61 ttctcctctc ttccctttca ctccatgttc ttctttttcg ccgtcgctcc ggttttcttc
      121 ttgctactcc cgccgcctct attctccggt taccgtctac gccgcgaaga aactctccca
      181 caaaatcagt tctgagtttg atcgcgacaa ggatccgatg gtgagcaagg gcgaggagct
      241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
      361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      421 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      721 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
      841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      901 cgggggtacc atgaccagca aggtgtacga ccccgagcag aggaagagga tgatcaccgg
      961 cccccagtgg tgggccaggt gcaagcagat gaacgtgctg gacagcttca tcaactacta
     1021 cgacagcgag aagcacgccg agaacgccgt gatcttcctg cacggcaacg ccactagcag
     1081 ctacctgtgg aggcacgtgg tgccccacat cgagcccgtg gccaggtgca tcatccccga
     1141 tctgatcggc atgggcaaga gcggcaagag cggcaacggc agctacaggc tgctggacca
     1201 ctacaagtac ctgaccgcct ggttcgagct tctgaacctg cccaagaaga tcatcttcgt
     1261 gggccacgac tggggcgccg ccctggcctt ccactacgcc tacgagcacc aggacaggat
     1321 caaggccatc gtgcacatgg agagcgtggt ggacgtgatc gagagctggg acgagtggcc
     1381 agacatcgag gaggacatcg ccctgatcaa gagcgaggag ggcgagaaga tggtgctgga
     1441 gaacaacttc ttcgtggaga ccgtgctgcc cagcaagatc atgagaaagc tggagcccga
     1501 ggagttcgcc gcctacctgg agcccttcaa ggagaagggc gaggtgagaa gacccaccct
     1561 gagctggccc agagagatcc ccctggtgaa gggcccatgg atgaaaactg tgaaagtgaa
     1621 tataacaacc cctgatgggc cagtctacga cgctgatatc gagatggtgt ccgtgcgggc
     1681 cgagagtggt gatctcggca tcctccccgg tcacattccc acaaaggccc cactgaagat
     1741 cggagctgtg cggctgaaga aggacggcca aaccgagatg gtcgcagtct caggcggcac
     1801 tgttgaagtg cggcctgacc acgttaccat taatgctcaa gccgctgaaa cagccgaagg
     1861 aatcgacaaa gagagagcag aagccgcaag acagagggcc caggagcggc tgaactctca
     1921 atccgatgac accgatattc gccgggccga gctggcactg cagagggccc tgaacagact
     1981 ggacgtggct gggaaggcaa acgagctcgg caagcccgac gtggtgcaga tcgtgagaaa
     2041 ctacaacgcc tacctgagag ccagcgacga cctgcccaag ctgttcatcg agggtgaccc
     2101 cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca ccgagttcgt
     2161 gaaggtgaag ggcctgcact tcctccagga ggacgccccc gacgagatgg gcaagtacat
     2221 caagagcttc gtggagagag tgctgaagaa cgagcagtaa gaattc
//
