LOCUS       sequence-80068-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pREP
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..51
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     239..261
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     253..270
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     254..270
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
       61 agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg
      121 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      181 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      241 cagtcacgac gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtcaacatg
      301 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      361 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      421 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      481 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      541 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
      601 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
      661 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      721 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      781 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      841 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      901 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      961 gtaagggatg acgcacaatc ccactatcct t
//
