LOCUS       sequence-80092-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN
  TITLE     The role of PACT in the RNA silencing pathway.
  JOURNAL   EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19.
  PUBMED    16424907
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2006-02-8"; volume: "25(3)"; pages: "522-32. Epub 2006 Jan 
            19"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-delHelicC
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccca ccatggacta caaggacgac gatgacaaga
      121 agcttatctt gagaaacaag tgttccaagt cggttgatac tggtgagact gacattgatc
      181 ctgtcatgga tgatgatgac gttttcccac catatgtgtt gaggcctgac gatggtggtc
      241 cacgagtcac aatcaacacg gccattggac acatcaatag atactgtgct agattaccaa
      301 gtgatccgtt tactcatcta gctcctaaat gcagaacccg agagttgcct gatggtacat
      361 tttattcaac tctttatctg ccaattaact cacctcttcg agcctccatt gttggtccac
      421 caatgagctg tgtacgattg gctgaaagag ttgtagctct catttgctgt gagaaactgc
      481 acaaaattgg cgaactggat gaccatttga tgccagttgg gaaagagact gttaaatatg
      541 aagaggagct tgatttgcat gatgaagaag agaccagtgt tccaggaaga ccaggttcca
      601 cgaaacgaag gcagtgctac ccaaaagcaa ttccagagtg tttgagggat agttatccca
      661 gacctgatca gccctgttac ctgtatgtga taggaatggt tttaactaca cctttacctg
      721 atgaactcaa ctttagaagg cggaagctct atcctcctga agataccaca agatgctttg
      781 gaatactgac ggccaaaccc atacctcaga ttccacactt tcctgtgtac acacgctctg
      841 gagaggttac catatccatt gagttgaaga agtctggttt catgttgtct ctacaaatgc
      901 ttgagttgat taca
//
