LOCUS       sequence-80093-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-pri-let-7d
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(454..472)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(455..472)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(492..509)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    498..722
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatccc gtttgtgact tgaggttgga
      121 ttttaagcaa atgtatttgt ggctttttac caaggtcata tggccagata acttttcaaa
      181 agcattagtt aaagaattct gattagtttg aattagaaac aaaactcaaa gaacatgacc
      241 taatttaaca ggttaatttg aagtgcatct gccaagtaga agaccagcaa gaaaaaaaaa
      301 atgggttcct aggaagaggt agtaggttgc atagttttag ggcagggatt ttgcccacaa
      361 ggaggtaact atacgacctg ctgcctttct tagggcctta ttattcaccg ataacctgtt
      421 tccttgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
      481 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      541 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      601 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      661 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      721 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgcccta
//
