LOCUS       sequence-80111-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-pri-let-7e
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(446..464)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(447..464)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(484..501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    490..714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(847..866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatcctc atccctggtc ctcctggtcc
      121 ctgtctgtct gtctgtcggg tctgtccacc tgccgcgccc cccgggctga ggtaggaggt
      181 tgtatagttg aggaggacac ccaaggagat cactatacgg cctcctagct ttccccaggc
      241 tgcgccctgc acgggacggg gcccggcggg gacccccagc cccactcagg gacccttagc
      301 cccactgggc tgccccaggg accctgggag gaagagccgg gctcttttct gtccttgtcc
      361 ctgcatcccc tccttcccct gaaatctgtt ttccttccct gtctgtctcc atctctgcct
      421 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      481 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      541 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      601 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
      661 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      721 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      901 aagctctaaa tc
//
