LOCUS       sequence-80113-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN
  TITLE     The role of PACT in the RNA silencing pathway.
  JOURNAL   EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19.
  PUBMED    16424907
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2006-02-8"; volume: "25(3)"; pages: "522-32. Epub 2006 Jan 
            19"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-delDUF
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccca ccatggacta caaggacgac gatgacaaga
      121 agcttattcc agagtgtttg agggatagtt atcccagacc tgatcagccc tgttacctgt
      181 atgtgatagg aatggtttta actacacctt tacctgatga actcaacttt agaaggcgga
      241 agctctatcc tcctgaagat accacaagat gctttggaat actgacggcc aaacccatac
      301 ctcagattcc acactttcct gtgtacacac gctctggaga ggttaccata tccattgagt
      361 tgaagaagtc tggtttcatg ttgtctctac aaatgcttga gttgattaca agacttcacc
      421 agtatatatt ctcacatatt cttcggcttg aaaaacctgc actagaattt aaacctacag
      481 acgctgattc agcatactgt gttctacctc ttaatgttgt taatgactcc agcactttgg
      541 atattgactt taaattcatg gaagatattg agaagtctga agctcgcata ggcattccca
      601 gtacaaagta tacaaaagaa acaccctttg tttttaaatt agaagattac caagatgccg
      661 ttatcattcc aagatatcgc aattttgatc agcctcatcg attttatgta gctgatgtgt
      721 acactgatct taccccactc agtaaatttc cttcccctga gtatgaaact tttgcagaat
      781 attataaaac aaagtacaac cttgacctaa ccaatctcaa ccagccactg ctggatgtgg
      841 accacacatc ttcaagactt aatcttttga cacctcgaca tttgaatca
//
