LOCUS       sequence-80139-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control for pCMV-calpainsensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Stockholm D, Bartoli M, Sillon G, Bourg N, Davoust J, Richard I
  TITLE     Imaging calpain protease activity by multiphoton FRET in living 
            mice.
  JOURNAL   J Mol Biol. 2005 Feb 11;346(1):215-22. Epub 2004 Dec 16.
  PUBMED    15663939
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-02-11"; volume: "346(1)"; pages: "215-22. Epub 
            2004 Dec 16"
COMMENT     Sequence Label: pCMV_eYFP-eCFP
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(4..717,718..720,721..723))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(49..70)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     398..417
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     657..678
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      720..729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
       61 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      121 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      181 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
      241 gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag
      301 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      361 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      421 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      481 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac
      541 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
      601 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
      661 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
      721 catggtggcg accggtggat cc
//
