LOCUS       sequence-80142-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pyronic, FRET sensor that allow quantitative measurement of pyruvate
            transport, pyruvate production and mitochondrial pyruvate 
            consumption in intact individual cells at high temporal resolution..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   San Martin A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito 
            R, Contreras-Baeza Y, Alegria K, Barros LF
  TITLE     Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for 
            Pyruvate.
  JOURNAL   PLoS One. 2014 Jan 21;9(1):e85780. doi: 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21.
  PUBMED    24465702
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-01-21"; volume: "9(1):e85780. doi"; pages: " 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21"
COMMENT     Sequence Label: Pyronic/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc gagcggccgc
      121 cactgtgctg gatatctgca gaattccacc acactggact agtggatccg aacatggtga
      181 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      241 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      301 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      361 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      421 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
      481 ccttcgagga caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct
      541 tcatctacga gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga
      601 agaagaccac cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga
      661 agggcgacgt caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca
      721 agaccatcta cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc
      781 gcatcgagat cctgaaccac ga
//
