LOCUS       sequence-80144-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pyronic, FRET sensor that allow quantitative measurement of pyruvate
            transport, pyruvate production and mitochondrial pyruvate 
            consumption in intact individual cells at high temporal resolution..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   San Martin A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito 
            R, Contreras-Baeza Y, Alegria K, Barros LF
  TITLE     Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for 
            Pyruvate.
  JOURNAL   PLoS One. 2014 Jan 21;9(1):e85780. doi: 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21.
  PUBMED    24465702
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-01-21"; volume: "9(1):e85780. doi"; pages: " 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21"
COMMENT     Sequence Label: Pyronic/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..711,712..714,715..717))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(43..64)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     392..411
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     651..672
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
       61 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
      121 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      181 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
      241 cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      301 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      361 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      421 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      481 catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg tcacgagggt
      541 gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca gcttgccgta
      601 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      661 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
      721 agatctctta agattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc
      781 ttcacgactt ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc
      841 gcgcgcttct tccggcttac cggccataat cgcttcaaa
//
