LOCUS       sequence-80145-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pyronic, FRET sensor that allow quantitative measurement of pyruvate
            transport, pyruvate production and mitochondrial pyruvate 
            consumption in intact individual cells at high temporal resolution..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   San Martin A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito 
            R, Contreras-Baeza Y, Alegria K, Barros LF
  TITLE     Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for 
            Pyruvate.
  JOURNAL   PLoS One. 2014 Jan 21;9(1):e85780. doi: 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21.
  PUBMED    24465702
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-01-21"; volume: "9(1):e85780. doi"; pages: " 
            10.1371/journal.pone.0085780. eCollection 2014 Jan 21"
COMMENT     Sequence Label: Pyronic/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(815..839)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcgcccttgc tcaccatggt agatctctta agattctttc gttgctccag acgacgcaga
       61 gaacgctcac ggcggctctc ttcacgactt ctgtcgagca aaatttcttc gataaaggcc
      121 agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat
      181 atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg
      241 aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga
      301 accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc gtttgattcc
      361 gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc acggatgcgt
      421 tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg
      481 tgtcgtgttt cgagcaagtc atactgtgac tcaggatggt cggagagcag ctccaccagc
      541 ggatcgctga aactttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga
      601 agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg
      661 tcaaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct
      721 tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt
      781 ttgctgtagg ccatggtacc aagggcgaat tcggagcctg cttttttgta caaacttgtg
      841 gtaccagatc tcttgtacag ctcgtccatg ccgtcggtg
//
