LOCUS       sequence-80186-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for aDTX-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-aDTX-lumitoxin
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             144..167
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             369..392
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             432..860
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             882..1028
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat gagcgccctg ctgatcctgg
      121 ccctggtggg cgccgccgtg gccgactaca aggacgacga cgacaagctg aagcttcagc
      181 ccagaagaaa gctgtgcatc ctgcacagaa accccggcag atgctacgac aagatccccg
      241 ccttctacta caaccagaag aagaagcagt gcgagagatt cgactggagc ggctgcggcg
      301 gcaacagcaa cagattcaag accatcgagg agtgcagaag aacctgcatc ggcggtaccg
      361 ccgccgccga ctacaaggac gacgacgaca agatcgacgc cgccgccggc ggcgccctgt
      421 gcaacgaatt cttggctact acacttgaac gtattgagaa gaactttgtc attactgacc
      481 caagattgcc agataatccc attatattcg cgtccgatag tttcttgcag ttgacagaat
      541 atagccgtga agaaattttg ggaagaaact gcaggtttct acaaggtcct gaaactgatc
      601 gcgcgacagt gagaaaaatt agagatgcca tagataacca aacagaggtc actgttcagc
      661 tgattaatta tacaaagagt ggtaaaaagt tctggaacct ctttcacttg cagcctatgc
      721 gagatcagaa gggagatgtc cagtacttta ttggggttca gttggatgga actgagcatg
      781 tccgagatgc tgccgagaga gagggagtca tgctgattaa gaaaactgca gaaaatattg
      841 atgaggcggc aaaagaactt agcggccgcg gtactcgagt tgctgtgggc caggacacgc
      901 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
      961 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga
     1021 aaccacgtag gattcgtatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1081 agttcatgcg cttcaaggtg cacat
//
