LOCUS       sequence-80192-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-FLAG-Lin28BdelN70
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(790..808)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(791..808)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(828..845)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatggactac aaagacgatg acgataaagg atccatggaa
      121 ggatttagaa gcctaaaaga aggagaacca gtggaattca catttaaaaa atcttccaaa
      181 ggccttgagt caatacgggt aacaggacct ggtgggagcc cctgtttagg aagtgaaaga
      241 agacccaaag ggaagacact acagaaaaga aaaccaaagg gagatagatg ctacaactgt
      301 ggtggccttg atcatcatgc taaggaatgt agtctacctc ctcagccaaa gaagtgccat
      361 tactgtcaga gcatcatgca catggtggca aactgcccac ataaaaatgt tgcacagcca
      421 cccgcgagtt ctcagggaag acaggaagca gaatcccagc catgcacttc aactctccct
      481 cgagaagtgg gaggcgggca tggctgtaca tcaccaccgt ttcctcagga ggctagggca
      541 gagatctcag aacggtcagg caggtcacct caagaagctt cctccacgaa gtcatctata
      601 gcaccagaag agcaaagcaa aaaggggcct tcagttcaaa aaaggaaaaa gacataacag
      661 gtcttcttca tatgttcttt cctttacccg gttgcaaagt ctacctcatg caagtatagg
      721 ggaacagtat ttcacaagca gtagctgacc tgggagcggc cgctcgagca tgcatctaga
      781 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      841 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctgga
//
