LOCUS       sequence-80239-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CalR1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..202
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggaagttctg ttccaggggc cccatatgca taccggtgaa accgaaagcg caccgggtcg
      241 tcgtgatcgt cgtaaacgtc gtacccgtgc agcactgacc ggtgcagcac tgcgtctggt
      301 tgcagaacgt ggtctggcac aggttaccgt tgaagaaatt tctgaagcag ccggtgttag
      361 tccgcgtacc ttttttaact atttcgcctg taaagatgat gccctggttg gtgatcatgc
      421 cggtgatgca gcacgtctgg ttgcccgtct ggcagcagtt ccgcctgaag ttccggttct
      481 gaccgcactg cgtgcagccc tggatgaagt tattgatgaa gttcgtaccg aacgtgaact
      541 gtggtgtctg cgtatggaag ttgttacccg taatccggtt ctgctgcctc gtctggttgc
      601 gggtaatgtt gaaaccgaac gtgcaattgc cgaagttgtt gcagcacgtg ttggtgttga
      661 tccggatcat ggttatccgg cactggttac cgcagttgcc ggtgcagcag ttcgtaccgc
      721 aatgacccgt tgggcagcac atccggatgg tccggatctg gcagatctgg ttgatgaagc
      781 atttgcagca ctggcagcag gtctgccgca gcctgatccg acctaaggat ccaagcttgc
      841 ggccgcactc
//
