LOCUS       sequence-80309-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-BarB
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..192
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggaagttctg
      181 ttccaggggc cccatatgac cccgaaacaa gaacgtgcat ttcgtacccg tacccagctg
      241 gttctgagcg cagcagaagc atttgatcgt cagggttttg caaccgcaag cctgaccgca
      301 attagcaata gtgccggtgt tagcaatggt gcactgcatt ttcactttga aagcaaagaa
      361 gcactggcag cagcagttga agccgaagca gcagaacgta tgcgtaccat tgttgatggt
      421 gcagcacgtc gtggtgcaag cgcactgcag gcactggttg ataccagcca tgcagttatg
      481 ctgcgtctgc gtcaggatgt tgttgttcgt gcaggttttc gtctgagcgg tgatgcagca
      541 cgtcaggcaa cccatgatct gccggaacat tggcgtcaga gcgttgttcg tctgctggaa
      601 cgtgcaggtc gtgatggtag cctgaccagc gcagttaccc cgagtgatgt tgccggtgtt
      661 gttaccgcaa ccgttctggg ttttggtgtt ctggcacgtt ttgatagcgc atggctggca
      721 agcggtagcc tgagcggttt ttggaaactg atgctgccga tgattgcagc aggtccggtt
      781 gaacgtgg
//
