LOCUS       sequence-80342-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-Dendra2 from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-Dendra2.W
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..796)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgaatc tgcagaattc gccctttcaa caggagtcca
       61 agctcagcta attaagcttt cacttgtaca cgccgctgtc gccggaccac acctggctgg
      121 gcagggggct gtagcgggcc acggcgtgct cgtacagctt caccttgttg tagtcgctgt
      181 cgttgcccag gatctcgatg cggtggtcca cgaagtgggc gtcgggcagc tgcaccacct
      241 tcttggcctt gtaggtggtc ttgaagtcgc acaggtagtg gccgccgccc tccagcagca
      301 gggccatgtt gatgttgccc accagcaggc cgtcgcgcac gtgcagcttc tcggtgctgg
      361 gctcccactt cagggtcttc ttctgcatca cggggccgtt gggggggaag ttggtgccct
      421 tgaagcgcac gttctggaag aagcagtcgc cctccaggct gatgtcgctg cggatggtgc
      481 agatgccctt gtcctcgaag gtcatggtgc gctcccagct gtagccctcg gggaagctct
      541 gcttgaagta gtcggggatg tcctcggggt acttggtgaa cacccggttg ccgtagtgca
      601 cggcggtggt caggatgtcg tagctgaagg gcaggggggc gccctccttc acggtcaggt
      661 tggcggtctg ggtgccctcg tagggcttgc ccttgccctc gccctcgatc acgaaggcgt
      721 ggccgttcac gttgccctcc atgtgcacct tcacgcgcat gtcctccttg atcaggttaa
      781 ttcccggggt gttcatggat cgatccttag cgctggtgta cttggtgatg gccttagtac
      841 cctcggacac ggcgtgcttg gccaactccc caggcagcag caggcgcacg gccgtctgga
      901 tctccctgga
//
