LOCUS       sequence-80358-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-136/63-VR-NG
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(63..83)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(63..81)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(102..118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(102..118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(115..137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(150..156,157..174,175..180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    195..216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc cagcttttgt
       61 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
      121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      241 ttccaccggt cgtctccacc cctgagcagg tagtggctat tgcatccaac ggagggggca
      301 gacccgcact ggagtcaatc gtggcccagc tttcgaggcc ggaccccgcg ctggccgcac
      361 tcactaatga tcatcttgta gcgctggcct gcctcggcgg acgacccgcc ttggatgcgg
      421 tgaagaaggg gctcccgcac gcgcctgcat tgattaagcg gaccaacaga aggatccccg
      481 agaggacatc acatcgagtg gcaggttccc aactcgtgaa gagtgaactt gaggagaaaa
      541 agtcggagct gcggcacaaa ttgaaatacg taccgcatga atacatcgaa cttatcgaaa
      601 ttgctaggaa ctcgactcaa gacagaatcc ttgagatgaa ggtaatggag ttctttatga
      661 aggtttatgg ataccgaggg aagcatctcg gtggatcacg aaaacccgac ggagcaatct
      721 atacggtggg gagcccgatt gattacggag tgatcgtcga cacgaaagcc tacagcggtg
      781 ggtacaatct tcccatcggg ca
//
