LOCUS       sequence-80361-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate array vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: pFUS2_a3a
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..83
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(98..103,104..121,122..128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     141..163
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     160..176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     160..176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             172..660
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLGVR
                     LMRRLRLAVVLQRRDWENIHASVNARVGNCQASNKTKGSVGRLGLSFYLLFVGERSPE"
     primer_bind     195..215
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        197..215
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(262..281)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(263..281)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(291..307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(501..517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      581..667
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtctagacg tctcgtcagg tgagaccacc ggtggaaagc gggcagtgag cgcaacgcaa
       61 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg
      241 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      421 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgccgg tctcggggtc
      481 aggttgatga gacgcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc
      541 catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      601 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      661 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cg
//
