LOCUS       sequence-80410-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CylA (fatty acyl-AMP ligase) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Nakamura H, Hamer HA, Sirasani G, Balskus EP
  TITLE     Cylindrocyclophane biosynthesis involves functionalization of an 
            unactivated carbon center.
  JOURNAL   J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p.
            Epub 2012 Nov 2.
  PUBMED    23106426
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja308318p";
            journalName: "J Am Chem Soc"; date: "2012-11-14- 14"; volume: "134";
            issue: "45"; pages: "18518-21"
COMMENT     Sequence Label: pET-28a-CylA
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgca tttgctacaa caagaaaatc
      121 tatctttgat agaaatcatc aattatagag cccaatatca acccgataaa aaagcctaca
      181 tctttctgca aaacggagaa gaagaatcag caagtctcac ctacggggag ttagacagaa
      241 gagcaagagc gatcgcagct cgtcttcaat cttggcaagg agagcgggct ttattgttat
      301 acccttctgg attagagttt attacagcct tctttggttg tttgtacgct ggggtagtgg
      361 cgatgccagt ttatcctccc aaacgtaatc aaaagttatc tcggttgctc tctatcgtta
      421 atgatgccca ggctaactta gcactgacaa cctcatcaat attgcttgac attagtcaaa
      481 agtggaaaga ggaaacagag tcattaaccc agttgaactg ggtgcctaca gatactattg
      541 aagctgattc tcaagatttt gtacctacat tagtaacacc tgagagtttg gcgtttttgc
      601 aatatacttc cggttctacg ggaacaccca aaggggtgat ggttactcat ggtaacctga
      661 tccacaattc ggagtgcatt aagcaagcat ttgaactcac atcagacagt gtatctgtga
      721 gttggttgcc cacttttcat gatatgggac tgattgatgg agtacttcaa cctttgtata
      781 ctggttttac tggttatcta atgccacctg tagcttttct catgaaaccg gcttgttggt
      841 tgcaggcaat tacccgatat a
//
