LOCUS       sequence-80516-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the codon optimized ORF1 of L1RP with his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM
  TITLE     Molecular reconstruction of extinct LINE-1 elements and their 
            interaction with nonautonomous elements.
  JOURNAL   Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. 
            Epub 2012 Aug 23.
  PUBMED    22918960
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/molbev/mss202"; journalName: "Mol Biol Evol"; date: 
            "2013-01"; volume: "30"; issue: "1"; pages: "88-99"
COMMENT     Sequence Label: pBudORF1-CH
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tgcattcctg caggtcgaca tcgatcttaa gcagtacttc tagaggatcc
      121 gaccaaaagc tagctaaaac cacaaagatg ggcaagaagc agaaccgcaa gaccggcaac
      181 agcaagaccc agagcgccag cccccccccc aaggagcgca gcagcagccc cgccaccgag
      241 cagagctgga tggagaacga cttcgacgag ctgcgcgagg agggnttccg ccgcagcaac
      301 tacngcgagc tgcgcgagga catccagacc aagggcaagg aggnggagaa cttcgagaag
      361 aacctggagg agtgcatcac ccgcatcacc aacaccgaga agtgcctgaa ggagctgatg
      421 gagctgaaga ccaaggnccg cgagctgcgc gaggagtgnc gcagcctgng cagccgctgc
      481 gaccagctgg aggagcgcgt gagcgcaatg gaggacgana tgaacganat gaagcgcgag
      541 ggcaagttcc gcgagaagcg catcaagcgc aacgagcaga gcctccagga gatatgggac
      601 tgcgtgaagc gccccaacct gcgcctgatc ggcgtgcccg agagcgacgt ggagaacggc
      661 accaagctgg agaacaccct
//
