LOCUS       sequence-80632-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Kwok SC, Kumar S, Dai G
  TITLE     Characterization of a Variant of ERGIC2 Transcript.
  JOURNAL   DNA Cell Biol. 2013 Dec 4.
  PUBMED    24303950
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Cell 
            Biol. 2013 Dec 4."
COMMENT     Sequence Label: pHTN HaloTag CMV-neo/ERGIC2-variant
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        249..606
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        249..440
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(272..292)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      457..592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(711..730)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
       61 ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac aaaatattaa
      121 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      181 catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
      241 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
      301 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      361 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      421 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      541 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      601 tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact taaggctaga gccaccatga
      661 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
      721 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
      781 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
      841 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
      901 acgttgtcac tgaagcggg
//
