LOCUS       sequence-80686-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71421
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(614..632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        700..804
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcagctttt
      301 atgttggtga tatggtggta aatgtaggga tttagtttac aattgcgtat gtctgtgttg
      361 gatatctgta gtgctgttct tatggcttag atcttgtaat ttctcattac agtatcaatg
      421 aatagatatc agtttctagt gatgacattg gttcgtcttt tagctgttga ttaatttttc
      481 ttaattgatt catcctattg caattcttct gaatttaaat tgtatactgt gaaattaaga
      541 aaattcttga aattaatgag aatttgagta atagcgcttg agaccacgaa gtggctcttc
      601 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      661 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      781 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      841 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg ttttttt
//
