LOCUS       sequence-80712-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by 
            rice snoRNA U3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGE31
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..102
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     94..111
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     95..111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(120..372)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(394..417)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    428..452
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(485..532)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
       61 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcccg
      121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      361 gtttgaacga tcggggaaat tcgagctcta agccttgtca tcgtcatcct tgtagtcgct
      421 gttatcaacc actttgtaca agaaagctgg gtctagatat ctcgagtgcg gccgcgaatt
      481 cttacttttt cttttttgcc tggccggcct ttttcgtggc cgccggcctt ttgtcgcctc
      541 ccagctgaga caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg
      601 tggcgtccag cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt
      661 acttgaaggc ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg
      721 cctgctctct gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat
      781 tagcgtcggc caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca
      841 ggtagtgctt gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct
      901 tcagcttctc atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca
      961 gttcgtttcc cttctgcagt tcgccggcag aggccagcat tctcttccgg ccgttttcca
     1021 gctcgaacag ggagtactta ggcagc
//
