LOCUS       sequence-80723-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC123SB-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        389..733
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     677..697
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
       61 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
      121 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
      181 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
      241 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      301 tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
      361 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      421 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      481 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      601 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
      661 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
      721 atttggagag gacctcgact ctagaggatc cccgggtacc gggccccccc tcgaggcgcg
      781 ccaagctatc aaacaagttt gtac
//
