LOCUS       sequence-80725-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        386..730
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     674..694
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    791..811
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
       61 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
      121 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
      181 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
      241 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
      301 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      361 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      421 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      481 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      541 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      601 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      661 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      721 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
      781 agctatcaaa caagtttgta caaaaaagca gg
//
