LOCUS       sequence-80787-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Chang HC, Lindquist S
  TITLE     Conservation of Hsp90 macromolecular complexes in Saccharomyces 
            cerevisiae.
  JOURNAL   J Biol Chem 1994 Oct 7;269(40):24983-8.
  PUBMED    7929182
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "1994-10-7"; volume: "269(40)"; pages: "24983-8"
COMMENT     Sequence Label: pTGPD-H-Hsp82
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..45
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     73..89
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     74..90
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        160..803
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     696..719
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             937..954
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaccatga ttacgccaag ctcgaaaatt aaccctcact aaagggaaca aaagctggta
       61 ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc tcatgtttga
      121 cagcttatca tcgataagct tggctgcagg tcgagtttat cattatcaat actgccattt
      181 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
      241 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
      301 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
      361 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
      421 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
      481 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
      541 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
      601 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
      661 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
      721 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
      781 aaacaccaag aacttagttt cgacggatcc gattcttaac atctgtgacc tcctcatttc
      841 ttcccgctgt attagagttc aagaaatcat acctgataga aaatagagtc ctataaacaa
      901 aagcacaaac aaacacgcaa acatagaatt catatgcacc accatcacca ccatatggct
      961 agcgaaact
//
