LOCUS       sequence-80926-e        5370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for mutated human GPR56 exon 1m promoter activity. It 
            contains a 15-bp deletion in a conserved noncoding element. The 
            mutated human GPR56 e1m promoter was inserted into pGL3E..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang 
            PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, 
            Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh 
            CA
  TITLE     Evolutionarily dynamic alternative splicing of GPR56 regulates 
            regional cerebral cortical patterning.
  JOURNAL   Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
  PUBMED    24531968
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-14- 14"; volume: "343"; 
            issue: "6172"; pages: "764-8"
COMMENT     Sequence Label: pGL3E-hGPR56 e1m promoter perisylvian polymicr...
FEATURES             Location/Qualifiers
     source          1..5370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             394..2046
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(460..478)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2087..2208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2098..2117)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2152..2171
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2530..2550
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2702..2719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2873..3461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2953..2972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3632..4423,4424..4492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4255..4274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4493..4597)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4624..5079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4711..4730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4921..4942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5210..5258
                     /note="synthetic polyadenylation signal"
     misc_feature    5272..5363
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5312..5331
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttcc
       61 ccataaatcg ctgtcctaac ccctgccctc cctcctgcca gctccctgtc tggcctgggc
      121 agcgtctgag ttgaggactt gggaacagga caagttacgg agccacgttg ctttgctggg
      181 tctgagccgg ggtgtgacgt agtccctgca gctgccaacg gttgccaggg gctgctgtca
      241 cctgcgcccc ttctcccgcg ctggcggctg gggcttctca gcctctattc cctggctgtc
      301 ccctttgttt gaagctccag tgagggagca gtggctgggg tggcccagct tcaaagtcaa
      361 gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa
      421 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
      481 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
      541 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
      601 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
      661 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
      721 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
      781 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
      841 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
      901 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
      961 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
     1021 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
     1081 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     1141 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     1201 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
     1261 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     1321 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     1381 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     1441 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     1501 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     1561 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     1621 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     1681 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     1741 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     1801 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     1861 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     1921 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     1981 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
     2041 gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     2101 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2161 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2221 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2281 aacctctaca aatgtggtaa aatcgataag gatctgaacg atggagcgga gaatgggcgg
     2341 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     2401 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     2461 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     2521 actttccaca ccctaactga cacacattcc acagcggatc cgtcgaccga tgcccttgag
     2581 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     2641 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     2701 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2761 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2821 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2881 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2941 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3001 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3061 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3121 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3181 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3241 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3301 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3361 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3421 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3481 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3541 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3601 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3661 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3721 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3781 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3841 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3901 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3961 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4021 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4081 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4141 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4201 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4261 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4321 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4381 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4441 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4501 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4561 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4621 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5041 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     5101 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     5161 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     5221 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5281 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5341 tgcaggtgcc agaacatttc tctatcgata
//
