LOCUS       sequence-80992-e        2340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-2NQ
FEATURES             Location/Qualifiers
     source          1..2340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(91..750)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     665..684
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(751..853)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(896..1306)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLVSTSNPFCQSQLLFKTAKFKELLNDVITKHIHHELVG
                     SLVRPSSNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNG
                     EWRLMRYFLLTHLCGICDGRSLRRINKSWCPC"
     primer_bind     1114..1136
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1128..1145
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1129..1145
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1302..1318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1302..1318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1315..1337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1350..1356,1357..1374,1375..1380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1395..1416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1533..1550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1784..1803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
       61 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
      121 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
      181 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatagtgaa
      241 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      301 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
      361 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      421 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      481 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaactccgg
      541 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
      601 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
      661 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
      721 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
      781 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      841 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
      901 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaaata ccgcacagat
      961 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     1021 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1081 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1141 ccagtgaatt cgagctcggt ctcaccaggc tgcctaccaa ctcatggtgg atgtgtttgg
     1201 taattacgtc attcagaagt tctttgaatt tggcagtctt gaacagaagc tggctttggc
     1261 agaacggatt cgaggtagag accaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcaaa gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
//
