LOCUS       sequence-81024-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 69-bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL815
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    101..275
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(304..320)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(304..320)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(348..365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(348..364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(357..379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    567..591
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
       61 acgtttttat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
      121 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
      181 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
      241 tatctgggaa ctactcacac attattatgg agaaactcga gaattcgagc tcggtacccg
      301 ggggatccac tagttctaga gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt
      361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      481 ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc
      541 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa c
//
