LOCUS       sequence-81059-e        8769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Transferrin receptor protein 1 ectodomain in 
            mammalian cells. N-terminal His tag, biotinylation sequence and rat 
            Cd4d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: TFRC-His-bio
FEATURES             Location/Qualifiers
     source          1..8769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2869..2924
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2923..2942)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5160..5178)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5246..5350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5351..5419,5420..6211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5569..5588)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6382..6970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6871..6890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7124..7141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7258..7279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7294..7299,7300..7317,7318..7324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7332..7348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7337..7359
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7356..7372
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7356..7372
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7643..8022
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8023..8226
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8177..8197
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8223..8247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8728..8747
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgccttgca agggcgtgga acccaagacc gagtgcgaga gactggccgg caccgagtct
      841 cccgtgcggg aagaacccgg cgaggacttt cctgccgcca gacggctgta ctgggacgac
      901 ctgaagcgga agctgagcga gaagctggac agcaccgact tcaccggcac catcaagctg
      961 ctgaacgaga acagctacgt gcccagagag gccggcagcc agaaggacga gaacctggcc
     1021 ctgtacgtgg aaaaccagtt cagagagttc aagctgagca aagtgtggcg ggaccagcac
     1081 ttcgtgaaga tccaggtcaa agacagcgcc cagaacagcg tgatcatcgt ggacaagaac
     1141 ggccggctgg tgtacctggt ggaaaaccct ggcggctacg tggcctacag caaggccgcc
     1201 accgtgaccg gcaagctggt gcacgccaac ttcggcacca agaaggactt cgaggacctg
     1261 tacacccccg tgaacggcag catcgtgatc gtgcgggctg gcaagatcac cttcgccgag
     1321 aaggtggcca acgccgagag cctcaacgcc atcggcgtgc tgatctacat ggaccagacc
     1381 aagttcccca tcgtgaacgc cgagctgagc ttcttcggcc acgcccacct gggcaccggc
     1441 gatccttaca cccccggctt ccccagcttc aaccacaccc agttcccccc cagccggtcc
     1501 tccggcctgc ccaatatccc cgtgcagacc atcagccggg ctgccgccga gaagctgttc
     1561 ggcaacatgg aaggcgactg ccccagcgac tggaaaaccg acagcacctg tcggatggtc
     1621 accagcgaga gcaagaacgt gaagctgacc gtgtccaacg tgctgaaaga gatcaagatc
     1681 ctgaacatct ttggcgtgat caagggcttc gtggaacccg atcactacgt cgtcgtcggc
     1741 gcccagagag atgcctgggg acctggcgcc gctaagtctg gcgtgggaac agccctgctg
     1801 ctgaagctgg cccagatgtt cagcgacatg gtgctgaagg acggcttcca gcccagccgc
     1861 agcatcatct tcgcctcttg gagcgctggc gacttcggct ctgtgggagc caccgagtgg
     1921 ctggaaggct acctgagcag cctgcacctg aaggctttca cctacatcaa cctggacaag
     1981 gccgtgctgg gcaccagcaa cttcaaggtg tccgccagcc ctctgctgta caccctgatc
     2041 gagaaaacca tgcagaatgt gaagcaccct gtgaccggcc agttcctgta tcaggacagc
     2101 aactgggcct ctaaggtgga aaagctgacc ctggacaacg ccgccttccc attcctggcc
     2161 tactccggaa tccccgccgt gtccttctgc ttctgcgagg acaccgacta cccctacctg
     2221 ggcacaacca tggacaccta caaagagctg atcgagcgga tccccgagct gaacaaggtg
     2281 gccagagccg ccgctgaggt ggcaggccag tttgtgatca agctgaccca cgacgtggaa
     2341 ctgaacctgg actacgagcg gtacaacagc cagctgctga gcttcgtgcg ggacctgaac
     2401 cagtaccggg ccgacatcaa agagatgggc ctgagcctgc agtggctgta cagcgccaga
     2461 ggcgacttct tccgggccac cagtcggctg accaccgact tcggcaacgc cgaaaagacc
     2521 gacagattcg tgatgaagaa actgaacgac cgcgtgatgc gggtggaata ccacttcctg
     2581 agcccctacg tgtcccccaa agagtccccc ttccggcacg tgttctgggg cagcggcagc
     2641 cataccctgc ccgccctgct ggaaaacctg aagctgcgga agcagaacaa cggcgccttc
     2701 aacgagacac tgttccggaa ccagctggcc ctggctacct ggacaatcca gggggctgcc
     2761 aatgccctga gcggcgacgt gtgggacatc gacaacgagt tctgaggcgc gcctgaattc
     2821 ttctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat
     2881 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     2941 ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg
     3001 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     3061 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     3121 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     3181 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     3241 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     3301 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     3361 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     3421 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     3481 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     3541 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     3601 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     3661 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     3721 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     3781 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     3841 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     3901 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     3961 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     4021 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     4081 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     4141 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     4201 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     4261 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     4321 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     4381 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     4441 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     4501 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     4561 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     4621 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     4681 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4741 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     4801 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     4861 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4921 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4981 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5041 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5101 agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg
     5161 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5221 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5281 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5341 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5401 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5461 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5521 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5581 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5641 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5701 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5761 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5821 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5881 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5941 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6001 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6061 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6121 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6181 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6241 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6301 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7141 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7201 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7261 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7321 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7381 cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     7441 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
     7501 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
     7561 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     7621 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     7681 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7741 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7801 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7861 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     7921 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     7981 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8041 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8101 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
     8161 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8221 agagctcgtt tagtgaaccg tcagatcctc actctcttcc gcatcgctgt ctgcgagggc
     8281 cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc
     8341 ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg agtccgcatc
     8401 gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc aaggtaggct
     8461 gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg aggtgctgct
     8521 gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga ggtgtggcag
     8581 gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt acttctgcgc
     8641 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gatctggcca
     8701 tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg
     8761 tccaagttt
//
