LOCUS       sequence-81076-e        8084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP reporter for rack-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Demarco RS, Lundquist EA
  TITLE     RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in 
            Caenorhabditis elegans axon pathfinding and cell migration.
  JOURNAL   PLoS Genet. 2010 Nov 18;6(11):e1001215. doi: 
            10.1371/journal.pgen.1001215.
  PUBMED    21124943
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2010-11-18"; volume: "6(11):e1001215. doi"; pages: "
            10.1371/journal.pgen.1001215"
COMMENT     Sequence Label: pEL481
FEATURES             Location/Qualifiers
     source          1..8084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3754..3782)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4530..4550
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(5524..5546)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5646..5665
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5743..5761)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5829..5933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5934..6002,6003..6794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6152..6171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6965..7553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7454..7473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7707..7724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7841..7862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7877..7882,7883..7900,7901..7907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7915..7931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7920..7942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7939..7955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7939..7955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcagct ggtgtcctta aatgcggtgg tttaatgagg gattcgtaaa
       61 aacgagcatc attgactgga tcctgtcgag gtttccgagg atccatctga ttctcctata
      121 aatataaaat atctagattg ttagtttttt cgaaggaaaa atataaaaaa taaaatttcg
      181 ggaaccatag taaggcaaaa agtacaaaat gtaaattttt tcgttgaaca gaagccatgt
      241 ggtcttccat tcagattaag acggcaaaga gagagtaagc gaaagaggac gagcgttcag
      301 ataatctcat aaatcagcat gtaatggggt gaatggggcg gagaaggagg agctcggatg
      361 cttcttttgt gtttatttat gtgaatagag aggtagaaga atatgtatat attcatcccc
      421 aagagatgtt agaggatttg cagattactt ttagattatt aatgaagata tgagaatata
      481 agagacgaaa agtacatgaa tacaacaatt tttctaaaaa atcacgaagg cactatcaac
      541 taactgatta ctccttacaa tgatgatatt taacaatttt tgcaacaatc tagaagtacc
      601 gcatgacaat accaattgaa aattgttaat tgttaaaatg ggagtcacat ataaaagaaa
      661 aactaggaca tttgtagtga aaatgtacag ctagaaggca taacttctgc cactcccgtt
      721 cttaatcaaa aattagcctc gattagtgtc tttatgaaag tttcgattgt tctgatcatc
      781 tacaactgaa aaaagggtcc catccagtca gttaccacca attgaattac aaagtgataa
      841 tgatgtcgtc gtgtgccgat aacaacgtct aatctctatt actatcagag gttgattata
      901 aacaatcagg ggcactagac acaggaaaga aaaggatgca aaagttgtga agaagggaat
      961 caaagtctga gggggggggg ggcgattaaa cgggcggata gtgaatgcgg cggccgcaca
     1021 cacacatagg gacgggcatt tggaggaatg tgactaggaa caacacagag gcgaagagga
     1081 caagaaataa atcggacagt ctgtgtagag gcaagaaatt ggaggaggaa aaggcgaggg
     1141 acggtgcgga aaagcaccgc caaaccactg cgaaccgatc aataatacaa cggaaggtgc
     1201 tgcgaagaaa taagagagga acggaaagtt gagacgcagg ttgattgggt taaagattgc
     1261 actgattgca gagaagacag agaatcgagt gaagattggc tcaggaaaga gttgattgtt
     1321 gacattccca ttggaaaaaa acagatatcg aaaaaatgtc taggagcaaa aaaatatttt
     1381 tcattatggg aattgttgtg atgttattgc tattgcaagg ttctgttgtt gtattgaaag
     1441 tcagctaaaa aatggggaat tattgaaaat atattgtatt caaaaattct tcaaaataaa
     1501 aatgactttt ttgaatgaaa aaaaaacttt aaaatagtga tgttggcata tcaacgtaaa
     1561 atttaagatg agaaggtatt aaacattata aggaaacaac tcgaagattt tcaagcacat
     1621 accaaagttt tcacggacaa ttggccaatt tctacaagaa gtcaacattt ttgtcgcttc
     1681 gtcaatttga aacccataaa agcatgacta agctgacagg aattccacga aaaagcaata
     1741 gatatgcgca aagaactagt ctcgtcagat ttttctggca atatctcatg cagtaatctt
     1801 aatatgtctt gcacacatgc aaccccggat gctgaaatgt taaactttaa aattcgagct
     1861 cgacacgatg aatttgaatg gatggagaat aattagaact aaacccacat tacattgatt
     1921 ttaatgtaaa aactaaagta tttttgcttt taaatgagaa aataataatt tttcgaagaa
     1981 atcaaaccag ttttcagaaa tattaatttt aaggctcaaa taaatggctc gctaaactcg
     2041 aaaacaactg tcacaataaa agctaaacat aaataattga ataaataaat aagtaaattt
     2101 aaaaaagtgt caaaaactgc ttcatagatg caaatttaca agaaaaagtt aatttgttcc
     2161 gtaaattcgt tagttggcaa agtgccaggt taaaggcgca tagactttcg ctgtggggtc
     2221 tcgcagcgac tcaccccttc tcctctgctg ctggctggct agtttgacgc gaatttttag
     2281 gacgattttt ccttttttct gtatttttct gttaaagcaa tgattttttc aacattttca
     2341 acatttaata attaaagtgg ctatgttttt aacataattc gaacaaaatt atggttttcg
     2401 ttcgctctat tctccaaaat tttcattttt ttatatggaa catttttatt tttagcagat
     2461 cgaacggggt tacacagata gcaaaatggt ccaagagcaa atgaagctta ccggaaccct
     2521 cgagggtcac accggatggg ttacccaaat cgccacctac acccgcaacg acaagaccac
     2581 tgtcctgtca tcttcccgcg gtaagtcaat taatataacg ttttcaaatt tatactcaca
     2641 tttttcagac aagactatcc ttgtttggga tgtcgacagc gttgctccag tcgatgaggg
     2701 accaatcggt cgcccagttc gttcgctcac tggacataac cacttcgtct ctgacgttgt
     2761 catctcctcc gacggacaat tcgctctttc tggatcctgg gacaagaccc tccgcctctg
     2821 ggacctcaac cagtaagtaa ttcaataaaa aaacattgtg caaacaattt tataggggag
     2881 tgagcacccg tcaattcatc tcccacacca aggatgttct ctctgttgcc ttctctgctg
     2941 acaacagaca aatcgtctct ggatcccgcg acaagtccat caagctgtgg aacactcttg
     3001 cccagtgcaa gtacaccatc actgtacgtt ttttcaaatt cttttatagg aagatttatc
     3061 gaaactattg tttaggatga ttgccacacc gattgggttt ccaccgttcg tttctctcca
     3121 tcgaaccgcg atccagtgat cgtctccgca ggatgggaca aggttgtcaa ggtctggaac
     3181 ctcggaaact gccgtctgaa gaccaaccac atcggtcaca ccggatacgt caacaccgtt
     3241 accgtctctc cagatggttc cctctgtgct tccggaggca aagacggaca agctatgctt
     3301 tgggacctca acgagggcaa gcacttgtac acccttccag gaaacgacgt catcaacgcc
     3361 atgtccttct ctccaaacag atactggctc tgcgccgctg tcggatcttc catcaagatc
     3421 tgggatctcg aggacaagaa ggagatcgag gagctcaagc cagaaatcgc cagctctgga
     3481 agctcaagag gatccagccc acagtgtatc tctttggcct ggtctcaaga cggacaaacc
     3541 ctcttcgccg gatacaccga taacatcatc cgagtgtacc aggtgtcgat ccgtgcttcc
     3601 aacctgcagg tcgactctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     3661 cgaatgatac taacataaca tagaacattt tcaggaggac ccttgagggt accggtagaa
     3721 aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     3781 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     3841 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atgggtaagt
     3901 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact
     3961 actttctgtt atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac
     4021 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     4081 gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc atacatattt
     4141 aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     4201 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     4261 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttgtaagt
     4321 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt caaaattaga
     4381 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     4441 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     4501 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     4561 attacacatg gcatggatga actatacaaa tagcattcgt agaattccaa ctgagcgccg
     4621 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     4681 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     4741 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     4801 tgctcccacc ccctattttt gttattatca aaaaaacttc ttcttaattt ctttgttttt
     4861 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     4921 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     4981 aaatctacac aatgttctgt gtacacttct tatgtttttt ttacttctga taaatttttt
     5041 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     5101 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     5161 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     5221 aattttcctg cttttgcttt ttgggggttt cccctattgt ttgtcaagag tttcgaggac
     5281 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     5341 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     5401 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     5461 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5701 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     5761 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5821 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5881 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5941 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6001 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6061 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6121 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6181 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6241 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6301 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6361 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6421 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6481 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6541 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6601 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6661 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6721 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6781 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6841 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6901 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6961 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7021 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7081 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7141 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7201 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7261 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7321 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     7381 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7441 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7501 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7561 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7621 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7681 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7741 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7801 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7861 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7921 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt
     7981 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
     8041 aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
