LOCUS       sequence-81085-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains an N-terminal tandem affinity purification tag (relevant to
            any system) within a construct designed for genome integration into 
            trypanosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Schimanski B, Nguyen TN, Gunzl A
  TITLE     Highly efficient tandem affinity purification of trypanosome protein
            complexes based on a novel epitope combination.
  JOURNAL   Eukaryot Cell. 2005 Nov;4(11):1942-50.
  PUBMED    16278461
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2005-11"; volume: "4(11)"; pages: "1942-50"
COMMENT     Sequence Label: pN-PURO-PTP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(530..549)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tcctaaagtc gaggaggttg actaagttcc
       61 caggtgtatt tcggaccggt gctgcagtcg tctcactgtc tacggtgatg gtccgccgcc
      121 acattttctc ttcgtctctg tagcatttag gaaccctcgt tacgggaaag atgcgtgaca
      181 cattgatgct actactatta ttaatactct actattatta ttattattat tatttttatt
      241 ttagttcccc accaccgtca ttattattat cattattatt atcaatatta ccattattat
      301 tattacgata atgagcgtta gtgctattac cgctgttgtt gccatctttg ttaccgtggt
      361 gctttttttc ctttggttca tgcaactgca gcccggcatc gtcctcccat acttgcactc
      421 ctgctcttcc ttcctatttg catttgccca cgtgccccac gcgtgcatca cataagcatc
      481 aaggaacaag caacagctat aaaacaagga atatctgcct ctttgacata tgaccgagta
      541 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      601 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      661 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      721 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      781 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      841 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      901 cctggccacc gtcgg
//
