LOCUS       sequence-81169-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of non-cleavable Mdm2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Oliver TG, Meylan E, Chang GP, Xue W, Burke JR, Humpton TJ, Hubbard 
            D, Bhutkar A, Jacks T
  TITLE     Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive 
            feedback loop.
  JOURNAL   Mol Cell. 2011 Jul 8;43(1):57-71. doi: 10.1016/j.molcel.2011.06.012.
  PUBMED    21726810
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.06"; journalName: "Mol Cell"; date: 
            "2011-07-8- 8"; volume: "43"; issue: "1"; pages: "57-71"
COMMENT     Sequence Label: pCMV-myc3-Mdm2 D367E
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          128..700
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             770..799
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             806..835
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             842..871
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa
      121 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
      181 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
      241 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
      301 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
      361 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
      421 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
      481 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
      541 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
      601 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
      661 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
      721 tgttgtgctg tctcatcatt ttggcaaaga attcctcgac ggatcgatgg agcaaaagtt
      781 aatctcagag gaagatctgg aattagaaca gaaactcata agcgaagagg acttagagct
      841 ggagcagaag ttgattagtg aggaggattt gggcgccgga tccgtcgacc acccagtgtc
      901 tagagaattc accatggtga ggagcaggca aatgtgcaat accaacatgt ctgtacctac
      961 tgatggtgct gtaaccacct cacagattcc agcttcggaa caagagaccc tggttagacc
     1021 aaagccattg cttttgaagt tattaaagtc tgttggtgca caaaaagaca cttatactat
     1081 g
//
