LOCUS       sequence-81177-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with canonical 
            C-terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermCan
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..42)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(58..645)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaagg gcccttaaaa
       61 gtttatctcg ccgttattaa atttccgtct cacttcctct aaggttaatg tgccggcttt
      121 aatcatttct ccaccaatta aaagctcttc tacactaaga acagctccat tacaattagt
      181 gatatgattt aatcgtgtaa gctgagcttt gtagtttcct ttaaagtgac cactcacaaa
      241 taaaaactta aattccgtta cagaagatgg atagactttc caccattcat tagggttgag
      301 atgtttgttt cgtgtttgat tttcttcgac atatcgctcc atttcatctg cttggccaat
      361 tggcagatta taacctccgc tataagcttt agtatccacg atcacaccgt aatcaatagg
      421 agatccgaca gtataaattg ctccgtccgg tttccttgat ccacccaaat gtttacctct
      481 atatccataa actttcataa aaaattccat taccttcatt tcaagaattc tatcctgagt
      541 ggaatttctg gcaatttcaa ttaattcaat atattcatga ggcacatatt tcaatttatg
      601 acgaagttca gatttcttct cctccagttc acttttgact agttgggatc ccgcgactcg
      661 atgggaagtt ctctcgggaa tccgccggtt ggttcttttg atcaatgcgg gagcatgagg
      721 cagacccttt ttgactgcat cgagcgcggg tcgtccacca agacatgcca acgccaccag
      781 atggtcattc gttaacgcag ccaacgcggg atcgggcctg gacaattggg ctacgatgga
      841 ctccaaggct ggccgtcctc cccca
//
