LOCUS       sequence-81204-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN2
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..153
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             160..180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag caccatggac tacaaagacc atgacggtga ttataaagat
      121 catgacatcg attacaagga tgacgatgac aagatggccc ccaagaagaa gaggaaggtg
      181 ggcattcacc gcggggtacc tatggtggac ttgaggacac tcggttattc gcaacagcaa
      241 caggagaaaa tcaagcctaa ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg
      301 gggcatggct tcactcatgc gcatattgtc gcgctttcac agcaccctgc ggcgcttggg
      361 acggtggctg tcaaatacca agatatgatt gcggccctgc ccgaagccac gcacgaggca
      421 attgtagggg tcggtaaaca gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg
      481 gcgggtgagc ttagggggcc tccgctccag ctcgacaccg ggcagctgct gcagatcgcg
      541 cagagagggg gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg
      601 gcccccttga acctgacccc agaccaggta gtcgcaatcg cgtcacatga cgggggaaag
      661 caagccctgg aaaccgtgca aaggttgttg ccggtccttt gtcaagacca cggccttaca
      721 ccggagcaag tcgtggccat tgcaagcaat gggggtggca aacaggctct tgagacggtt
      781 cagagacttc tcccagttct ctgtcaagcc cacgggctga ctcccgatca agttgtagcg
      841 attgcgtcca acggtggagg gaaacaag
//
