LOCUS       sequence-81225-e         422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: Transgene Module (TrZ)
FEATURES             Location/Qualifiers
     source          1..422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    374..393
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ctcgcccttg ctcaccatgg ttgtgctgca ggtcgactct agaggatcaa ttccttagct
       61 cctgagcggc cgcaagcttg gttggaatct aaaatacaca aacaattagg atccctgagg
      121 agcagttctt tgatttgcac caccaggagc ctcaaatttt tcaataaatt cacctgactg
      181 cacattagga ctttggcttt gagcttccca cctctcagtt acttgctgaa tactgtctgc
      241 ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc aacccttccc tgttggctac
      301 ttgtctcacc attgtaggcc taatgggaga cggatccgta gtttaacaca ttatacacta
      361 gggtcgactg tggaattgtg agcgctcaca attccacaac ctagggtcga ctgtggaatt
      421 gt
//
