LOCUS       sequence-81268-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUC21 (Amp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUC21-deltaBB
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..38
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     22..38
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(52..71)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(53..71)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(78..95)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(78..94)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(394..413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     526..547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(701..720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     820..842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(880..898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgatatcgcc gtggcggccg ctctagaact agtggatcga tccccaattc gccctatagt
       61 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
      301 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      361 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
      421 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggtgggcca tcgccctgat
      481 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      541 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
      601 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      661 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
      721 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      781 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
      841 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
      901 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca tt
//
