LOCUS       sequence-81301-e        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S and C699T)
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..231
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     182..202
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     276..295
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        276..294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             308..340
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1095..1113)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1096..1113)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1133..1150)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1139..1347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
       61 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      121 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      181 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      241 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      301 caagcttatg gctagcatga ctggtggaca gcaaatgggt ggatccgagt cctttgggct
      361 gttctccagc gtcatcaatg gggaggagca tgagcaaacc catcgggcta tattcaggtt
      421 tgtgcctcgg catgaagatg aacttgagct ggaagtggat gaccccctgc tggtggagct
      481 gcaggcagaa gactattggt atgaggccta taacatgcgc accggagccc gcggggtctt
      541 ccctgcctac tatgccattg aggtcaccaa ggagcctgag cacatggcag cccttgccaa
      601 aaacagcgac tggattgacc agttccgggt gaagttcctg gggtctgtcc aggttcctta
      661 tcacaagggc aatgatgtcc tctgtgctgc tatgcaaaag atcgccacca cccgccggct
      721 caccgtgcac tttaacccgc cctccagctg tgtccttgag atcagtgtca ggggtgtcaa
      781 gataggcgtc aaagctgatg atgctctgga ggccaaggga aataaatgta gccacttctt
      841 ccagctaaag aacatctctt tctgtggata ccatccaaag aataacaagt actttgggtt
      901 tatcactaag caccctgctg accaccggtt tgcctgccat gtctttgtgt ctgaagattc
      961 caccaaagcc ctggcggagt ctgtggggcg tgcatttcag cagttctaca agcagtttgt
     1021 ggagtatacc actcctacag aagatatcta cttggagtag gcggccgctc gagcatgcat
     1081 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     1141 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     1201 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     1261 agtaggtgtc attctattct gggggggtgg ggtggggcag gacagcaagg gggaggattg
     1321 ggaagacaat agcaggcatg ctgggga
//
