LOCUS       sequence-81368-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Phamret, a monomeric photoconvertible 
            fluorescent protein, fused to Fibrillarin for nucleolar 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Matsuda T, Miyawaki A, Nagai T
  TITLE     Direct measurement of protein dynamics inside cells using a 
            rationally designed photoconvertible protein.
  JOURNAL   Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 
            2008 Mar 16.
  PUBMED    18345008
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1193"; journalName: "Nat Methods"; date: "2008-04"; 
            volume: "5"; issue: "4"; pages: "339-45"
COMMENT     Sequence Label: Phamret-Fibrillarin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(138..159)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     487..506
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
       61 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      121 tcggtaccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      181 tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      241 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc gtcctcgatg
      301 ttgtggcgga tcttgaagtg ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      361 acgttgtggc tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      421 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      481 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tacgctcctg gacgtagcct
      541 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      601 cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      661 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      721 gacacgctga acctgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      781 ccggtgaaca gctcctcgcc ct
//
