LOCUS       sequence-81412-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid for integration of target sequence at HO locus, Kan 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: HO-Poly-KanMX4-HO
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..37)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21..37)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(607..804)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatagggcga attggggatc gatccactag ttctagagcg gccgcatagg ccactgtaag
       61 attccgccac attttataca ctctggtcct ttaactggca aaccttcggg cgtaatgccc
      121 aatttttcgc ctttgtcttt tgcctttttc acttcacgtg cttctggtac atacttgcaa
      181 tttatacagt gatgaccgct gaatttgtat cttccatagc atctagcaca tactcgattt
      241 ttaccactcc aatctttata aaaatacttg attccctttc tgggacaagc aacacagtgt
      301 tttagattct ttttttgtga tattttatgc tgttctccca cacagcagcc tcgacatgat
      361 ttcacttcta ttttgttgcc aagcaagaaa tttttatggc cttctatcgt aagcccatat
      421 acagtactct caccctggaa atcatccgtg aagctgaaat atacgggttc cctttttata
      481 attggcggaa cttctcttgt tttgtgacca cttcgacaat atgacaaaac attctgtgaa
      541 gttgttcccc caggaattcg agctcgtttt cgacactgga tggcggcgtt agtatcgaat
      601 cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta ctttctgcgc
      661 acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac
      721 atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca agttcttgaa
      781 aacaagaatc tttttattgt cagtactgat tagaaaaact catcgagcat caaatgaaac
      841 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
      901 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
      961 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtc
//
