LOCUS       sequence-81426-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single component LSL vector. Contains 2x35S:Zif268:FokI followed by 
            the us:NPTII template. This sequence is between the LIR and SIR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ.D.R
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(822..842)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(876..900)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(961..1007)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(996..1016)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acttcctcaa gtgtcaatgt accagcttta atcatttctc caccaatcaa aagctcttct
       61 acactaagaa cagctccatt acaattagtg atatgattca atcttgtaag ctgagctttg
      121 tagtttcctt tgaaatgacc actcacaaac aagaacttaa attctgttac tgaagatgga
      181 tagactttcc accattcatt agggttgata tgcttgtttc ttgtttgatt ctcttcgaca
      241 tatctttgca tttcatctgc ttgaccaatt ggaagattat aacctcctga atatgcctta
      301 gtatcaacga tcacaccgta atcaatagga gatccgacag tataaattgc tccgtctggt
      361 ttccttgatc cacccaaatg tttaccacga taaccataaa ccttcataaa gaactccatc
      421 accttcattt caaggattct atcctgagtt gaatttcttg cgatttcaat caattcaata
      481 tattcatgtg gcacatattt caatttatgt ctaagttcag atttcttctc ttccaattca
      541 gatttcacta gttccttctg acgaagatgg atctttgtat gacgcttcct ttcatcactt
      601 cttgcgaact tacgaccaca gatgtcacat gcgaatggtt tctcaccagt atgggttctg
      661 atatgtgtag tcaaatgatc gctacgacta aagttcctca tacagattct acactggaaa
      721 ggcttctgtc ctgtgtggat tctaatatgc ctagtgagtt cgtctgacct actaaaacgt
      781 ctatcacaag actcaactgg acaagcgtaa ggcaattcaa gaacctttct ctttttcttt
      841 ggaggggagg aagccatggt gaagggcgaa ttcggagcct gcttttttgt acaaacttgt
      901 ttgatagctt ggcgcgcctc gagggggggc ccggtacccg gggatcctct agagtcgagg
      961 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     1021 attgtgcgtc atcccttacg tcagtggaga tatcacat
//
